Modifying Enzymes
New England Biolabs, Inc. mRNA Decapping Enzyme – 2000 units
mRNA Decapping Enzyme catalyzes the removal of 7-methylguanosine cap (m7G) from 5 end of mRNA, producing 5 monophosphate and releasing m7GDP. mRNA Decapping Enzyme is capable of decapping mRNAs of various lengths and removes both Cap0 and Cap1 structures with similar efficiency. mRNA Decapping Enzyme also converts 5 triphosphate ends to 5 monophosphate, albeit with reduced efficiency. 5' monophosphorylated RNA can be exploited in a variety of downstream applications, including 5' RNA Ligase-mediated RACE, RNA-seq, and 5' -> 3' exonuclease digestion.
Sigma Aldrich Fine Chemicals Biosciences N Glycosidase f recombinan
Peptide-N-glycosidase f Peptide-N4-(acetyl-beta-glucosaminyl)-asparagine amidase
New England Biolabs, Inc. Thermostable Inorganic Pyrophosphatase – 250 units
Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate: P2O7-4 + H2O -> 2HPO4-2.
Aldevron SpyFi Cas9 Nuclease, 0.25mg
0.25mg dispense of SpyFi Cas9 Nuclease. From basic research to clinical applications, our SpyFi Cas9 Nuclease delivers greater editing efficiency and reduced off-target effects without loss of on-target activity to help accelerate your CRISPR-Cas9 gene editing program. SpyFi Cas9 Nucleases are available at Research-Grade through Fisher, or across all quality grades (Research Grade or GMP) directly through Aldevron.
SpyFi Cas9 Nuclease is uniquely positioned to satisfy a wide range of needs from basic research to clinical applications. When used in combination with effective guide, target site and double-stranded DNA cut strategies, SpyFi Cas9 Nuclease delivers greater editing efficiency, higher fidelity and less off-target activity than wild-type SpCas9 nuclease.
Functional Performance
•Reduced off-target effects
•Ready to use for electroporation or transfection
•10 mg/mL formulation for ease of use
Bioworld Cellulase (Onozuka R-10), 5 g
Cellulase (Onozuka R-10) From Trichadema Viride. Enzyme mixture often used in combination with Macerozyme R-10 (M22010) for isolation of plant protoplasts. One unit of Cellulase will liberate 1.0 µmole of glucose from carboxymethyl cellulose.Optimum pH range: 4-5.Soluble in water (10mg/ml)Contents:Cellulase: 1.0 U/mg Pectinase: 0.4 U/mg Hemicellulase: 1.0 U/mg alpha-Amylase: 0.6 U/mg Protease: 0.01 U/mg
INTACT GENOMICS INC Exonuclease III 25000 Units (100 units/µl)
Exonuclease III (Exo III) is an exodeoxyribonuclease that digests one strand of duplex DNA from a blunt end, 5′ overhang or nick. The enzyme acts in a 3′→5′ direction producing stretches of single-stranded DNA (1, 2). Exo III is not active on 3′ overhangs of 4 or more bases in length and ssDNA. The enzyme has also RNase H, 3′-DNA phosphatase, and apurinic DNA endonuclease activities. Exo III digestions proceed at a uniform rate yielding predictable and reproducible results. However, the observed rate of nucleotide excision can vary depending on the individual characteristics of the reaction mix such as reaction temperature, ionic strength, template base content, template helical structure, and enzyme-to-DNA ratios.Applications• 3′ → 5′ exonuclease• Production of unidirectional nested deletions• Production of intermediates for site-directed mutagenesis• Preparation of strand-specific probes• Preparation of single-stranded substrates for dideoxy sequencing
Cayman Chemical Oleoyl SerotonIn-d17 1mg
An internal standard for the quantification of oleoyl serotonin by GC- or LC-MS
Medchemexpress LLC Catalase, aspergillus niger | 9001-05-2 | 500 MG
Catalase, Aspergillus niger is a key enzyme involved in the metabolism of H2O2 and reactive oxygen species (ROS), with its expression and localization being significantly altered in tumors. It functions as a free oxygen radical scavenger.
- Produced through fermentation by Aspergillus niger.
- Effective within a pH range of 4.0 to 10.0.
- Effective within a temperature range of 35°C to 40°C.
- Can be dissolved using water or as specified in relevant references.
- The active site contains an iron atom.
- Contains a N-terminal arm with a distal histidine, essential for the catalase reaction.
- Features a β-barrel domain with eight β-barrels arranged antiparallel with six α-helical insertions, forming the hydrophobic core.
- Includes a connection domain containing a tyrosine residue that binds the heme group.
- Has α-helical domains important for NADPH binding.
Sigma Aldrich Fine Chemicals Biosciences Superoxide Dismutase from bovine liver | 9054-89-1 | MFCD00132404 | 3ku
Superoxide Dismutase from bovine liver | Mol Wt: 32.5 kDa | 9054-89-1 | MFCD00132404 | 3ku
Amsbio LLC Heparinase III (EC 4.2.2.8) from Flavobacterium heparinum/ 0.5 IU
Heparinase III (EC 4.2.2.8) from Flavobacterium heparinum 0.5 IU
Enzo Life Sciences p300 (catalytic domain) (human), (recombinant) (100 µg)
Synonyms: E1A binding protein p300, Histone acetyltransferase p300. MW: 45.1 kDa. UniProt ID: Q09472. Source: Produced in E. coli. Catalytic domain (aa 1284-1673) of human p300. Purification: Partially purified by single-step affinity chromatography and gel filtration. Formulation: Liquid. In 50mM TRIS/HCl, pH 8.0, containing 0.1mM EDTA, 10% glycerol.
LifeSensors SUMO PROTEASE 2 500 UNITS
For removal of SUMOpro3 tags from fusion proteins expressed from the following SUMOpro3 expression systems: (Cat.#s: 1010A, 1016, 1010K, 1011, 2151). SUMO Protease 2, a highly active and robust recombinant protease, cleaves hSUMO3 from recombinant fusion proteins. Unlike thrombin, EK, or TEV protease, which recognize short, linear sequences, SUMO Protease 2 recognizes the tertiary structure of huSUMO3. As a result, SUMO Protease 2 will not cleave within the fused protein of interest. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.
GeneCopoeia BIOTIN PROTEIN LIGASE
Biotin Protein Ligase - 40 ug: 2 vials of 20 ug each (200,000 Units)
Enterprise Technology Solutions Heavy Duty Aluminum Foil Roll, 12" x 500 ft, Silver
Heavy Duty Aluminum Foil Roll, 12" x 500 ft, Silver